A stability indicating, simple, economic, rapid, precise and sensitive reverse phase high performance liquid chromatography method was developed and validated for determination of Alfuzosin in pharmaceutical dosage form. The quantitative determination of Alfuzosin was performed on an Agilent TC-C18 (2) (250 mm X 4.6 mm, 5 µm), stainless steel column with mobile phase consist of Water: Methanol (55:45 %v/v) pumped at a constant flow rate of 1 mL min-1. The detection is carried out using variable wavelength UV-Vis detector set at 245 nm. The method shows good peak shape, minimal tailing, with retention time 3.96 min for alfuzosin. The drug was subjected to oxidation, hydrolysis, and acidic, thermal and neutral hydrolysis to apply stress conditions. The developed method was able to separate degradation product generated under forced degradation studies. The developed method was validated as per ICH guidelines for the parameters such as specificity, linearity, accuracy, precision, limit of detection, limit of Quantitation and found to be satisfactorily. The response was linear in the drug concentration range of 20-100 µg mL-1 (r2 = 0.998). The proved to be suitable for stability testing, homogeneity testing and quality control of this compound in pharmaceutical dosage form.
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